Neuron counting

Useful if cells are well separated but won't work for images were many cell bodies are touching. This problem is, naturally, difficult to solve.

GUI not particularly user friendly since it isn't obvious that buttons need to be pressed in a particular order. I can't see how filtering parameters can be changed from within the GUI.

Thanks, I had looked at that link in the past. My problem is rather difficult because the cell bodies as small so I have few pixels to work with. Although they are touching, the pears are fairly easy to isolate because each pear is composed of a large number of pixels.

Your new GUI isn't working for me. It doesn't check whether the input is of the right type. e.g. my image was an int8 and under those circumstances the threshold slider doesn't work. I edited the code to convert the image to a double after loading but then something else broke.

You will find other users of this site consider it bad form for an author to rate their own submissions.

have you tried distance transformation before WST? there is a Matlab function BWout=bwdist(BWin).
i got this from my class notes by Prof. Alex Hartov